IMPACT OF SELENIUM NANOPARTICLES ON GROWTH BIOCHEMICAL CHARACTERISTICS AND YIELD OF BLACK GARAM VIGNA MUNGO

The present study aimed at the impact of selenium nanoparticles on the growth, biochemical characteristics and yield of Black gram Vigna mungo. Selenium nanoparticles were synthesized using the precipitation method and characterized by UV-VIS, SEM, EDAX and FT-IR. Physico-chemical parameters of soil and cow dung were estimated. Pot culture studies of black gram in different quantity of selenium nanoparticles such as 10,20,30,40 and 50mg for treatments such as T0(Control) T1,T2,T3,T4, and T5 respectively were conducted for 60 days. Growth characteristics such as seed germination efficiency (%), shoot length, root length, total fresh weight, total dry weight, leaf area index, and vigor index (%), biochemical characteristics such as chlorophyll a, b and total chlorophyll, anthocyanin, carotenoids, total protein, total soluble sugars, carotenoids, L- proline were measured after 60 days. Selenium nanoparticles were analyzed between 200-800nm wavelengths in UV-Vis Spectrophotometer. SEM image of selenium nanoparticles was observed as spherical in shape. EDAX spectrum recorded on purity of selenium nanoparticles. The FT-IR spectrum of selenium nanoparticles was analyzed in the range of 4000 - 400cm-1 spectral bands. Among the treatments the germination percentage, shoot length, root length, fresh and dry weight, leaf area and vigor index were higher in T3 containing 30mg of selenium nanoparticles. The chlorophyll a, b and total chlorophyll, total protein, total soluble sugar and L-proline were higher in T3. Number and weight of black gram were higher in T3.