HLA- B27 DETERMINATION BY BD FACS VIA SYSTEM- FLOW CYTOMETRY

HLA- B27, determination plays an important role in the diagnosis of certain Auto-immune disorders conditions like Ankylosing Spondylitis, SLE, Auto-immune pancreatitis. The main objective of the study is to estimate the levels of HLA-B27 by flow cytometry analysis, in the EDTA WB sample from the patients who are suffering with auto-immune disorders. The BD FACS Via system , is the flow cytometry technique ( four colour panel) used to determine the HLA-typing. The technique is quantitative and also screening method, used to state whether the auto-immune co infection is associated or not. The principle used is fluorescence labelled antibody binding technique to the blood cell antigen. The scatter plot and histogram curve is also derived with reference to the normal mean value. Here the study mainly involved a group of 25 people who are suffering from the auto-immune conditions, for the demonstration of HLA- B27 in the EDTA EB.