IDENTIFICATION OF HAPLOTYPES OF SITOPHILUS ZEAMAYS POPULATIONS IN THREE AGROECOLOGICAL ZONES

Storage systems are made vulnerable by insects that reduce the value of foodstuffs. In the case of maize, for example, grain losses can be substantial if nothing is done.The aim of this study is to identify the different haplotypes circulating in three agro-ecological zones of Senegal, such as the groundnut basin, eastern Senegal and the middle Casamance, and also to determine the demographic evolution of the populations obtained.To achieve this, samples from all three areas were subjected to mass rearing. Each population was constituted by an average of ten individuals from each sampled locality. DNA extraction, Polymerase Chain Reaction (PCR) and sequencing were carried out to obtain sequences. Parameters such as neutrality indices, mismatches distribution, and the haplotype network were determined using genetic software. The results obtained enabled us to identify 14 haplotypes circulating in these three agro-ecological zones, 10 of which are individual. Haplotype (H1) is the most represented and is common to all three zones. The results show a certain influence of the zones on the demographic structure of the populations studied.