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ISOLATION OF THE ENDOPHYTIC BACTERIA IN Andrographis paniculata Nees GROWING WILD IN HAU GIANG PROVINCE

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Author: 
*Huynh Van Truong, Hoang Minh Tu, Huynh Gia Bao, Tran Doan Hau, Lam Quang Nhut, Doan Van Dien
Country: 
Vietnam
Volume & Issue: 
Volume 05, Issue 06, June 2023
Page No: 
4702-4705
Abstract: 

Background: According to studies the endophytic bacteria, Andrographis paniculata Nees has antibacterial properties. Therefore, the isolation of the endophytic bacteria strains in Andrographis paniculata Nees was carried out in order to find some antibacterial strains that contribute to replacing synthetic antibiotics. Research subjects and methods: Eighteen of the endophytic bacteria strains were isolated from the roots, stems and leaves of Andrographis paniculata. Results: The results of investigation of antibacterial ability on 3 strains of Escherichia coli, Aeromonas hydrophila và Staphylococcus aureus showed that 6 strains were resistant to Escherichia coli, 4 strains were resistant to Aeromonas hydrophila and 3 strains were resistant to Staphylococcus aureus bacteria. Of which, 2 strains were resistant to both Escherichia coli and Aeromonas hydrophila strains, The strain was resistant to both Escherichia coli and Staphylococcus aureus strains. Conclusion: Two strains of bacteria TR2 and TL3 were identified as Enterobacter ludwigii CSR19B with 96% similarity and Kosakonia radicincitans DSM 16656 with 95% similarity.

KeyWords: 
Enterobacter ludwigii, antibacterial, Kosakonia radicincitans, the endophytic bacteria, Andrographis paniculata Nees.